Ethidium bromide solution msds sheet


Aqueous solutions of ethidium bromide (>1.0(g/ml) may be disposed of in one of two ways: 1) The ethidium bromide can be adsorbed onto an ion exchange column specifically for this purpose, available from Merck (catalogue number 437212Y). These columns will adsorb at least 300mg of ethidium bromide; the waste liquid may then be discarded to drain. Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Start studying BIOL 301 - Lab 1: safety and techniques. Learn vocabulary, terms, and more with flashcards, games, and other study tools. MIOSHA Regulations Covered in this Training Session ... ethidium bromide, phenol, silane ... Material Safety Data Sheets